DNA

Part:BBa_K5507006:Design

Designed by: josiazhang josiazhang   Group: iGEM24_HW-ShanghaiUnited   (2024-09-29)


gdh-3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The production of gluconate will reduce the amount of glucose in the medium and affect the production of bacteria cellulose (BC). Comparative studies have been carried out and some isolates that are more capable of producing cellulose from glucose have been found to have lower GDH activity. Therefore, we will knock out the gdh gene to achieve an increase in BC yield.


Source

gdh-3 is derived from the K. xylinus genome.

References